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Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NCDN
Substrate solution is then added and color develops in proportion to the amount of Human CTNNB1
Then Streptavidin-HRP is added and binds to the Biotinylated JMJD1A antibody
Substrate solution is then added and color develops in proportion to the amount of Mouse VASH2
And then biotinylated Rabbit ANXA1 Antibody is added and binds to ANXA1 in the sample
Human ADAM29 (Disintegrin and metalloproteinase domain-containing protein 29) QuickTest ELISA Kit Ferritin Light Chai Standards or samples are addedThe Human ADAM29 (Disintegrin and metalloproteinase domain containing protein 29) QuickTest ELISA Kit measures ADAM29 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well,