Human HUWE1 (E3 ubiquitin-protein ligase HUWE1) QuickTest ELISA Kit Packaging:96 wells Then a biotinylated detection antibody

Then a biotinylated detection antibody specific for Human SIGLEC3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated

CYTL1 present in the sample is added and binds to antibodies coated on the wells

Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to TGF-b1

Substrate solution is then added and color develops in proportion to the amount of Human MDH2

And then biotinylated Human FZD1 Antibody is added and binds to FZD1 in the sample

Human HUWE1 (E3 ubiquitin-protein ligase HUWE1) QuickTest ELISA Kit Packaging:96 wells Then a biotinylated detection antibodyThe Human HUWE1 (E3 ubiquitin protein ligase HUWE1) QuickTest ELISA Kit measures HUWE1 in Human Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and