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The concentration of Human LEP in the samples is then determined by comparing the OD of the samples to the standard curve
When the sample containing CTLA-4 is added to the plate
LGALS9 present in the sample is added and binds to antibodies coated on the wells
The Mouse (MCP-5) Monocyte Chemotactic Protein 5 ELISA Kit measures Monocyte Chemotactic Protein 5 in Mouse samples
And then biotinylated Mouse PLA2G2A Antibody is added and binds to PLA2G2A in the sample
Human LEPR (Leptin Receptor) ELISA Kit Packaging:96 wells The concentration of Human LEPThe Human (LEPR) Leptin Receptor ELISA Kit measures Leptin Receptor in samples. The plate has been pre coated with Human LEPR antibody. LEPR present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human LEPR Antibody is added and binds to LEPR in the sample. Then Streptavidin HRP is added and binds to the Biotinylated LEPR antibody. After incubation unbound Streptavidin HRP is washed away during a washing
