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TSHB in the sample or standard competes with a fixed amount of biotin-labeled TSHB for sites on a pre-coated antibody specific to TSHB
it binds with C4BPα bound to precoated antibody
The plate has been pre-coated with Human MAP1LC3B antibody
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VEGF-A
Substrate solution is then added and color develops in proportion to the amount of Human NOX1
Rat TSLP (Thymic Stromal Lymphopoietin) QuickTest ELISA Kit Packaging:96 wells TSHB in the sample orThe Rat TSLP (Thymic Stromal Lymphopoietin) QuickTest ELISA Kit measures TSLP in Rat Serum, Plasma, Cell Culture Supernatant, Cell Lysate or Tissue Lysate. This kit was based on sandwich ELISA method. The experiment lasted 120 minutes. Capture antibody was conjugated to an affinity tag that was recognized by a specific antibody coated on the QuickTest plate. Add the Cap Det Ab working solution into each well, then add the standards and pilot samples
